Journal: Neural Regeneration Research
Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions
doi: 10.4103/NRR.NRR-D-24-00676
Figure Lengend Snippet: OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.
Article Snippet: Bright field images were taken at 4× and 60× magnification using an Olympus upright fluorescence microscope (BX53) (Poh et al., 2021).
Techniques: CCK-8 Assay, Expressing, Control, Concentration Assay, Fluorescence, Microscopy, Knockdown, Electron Microscopy, Membrane, Cell Counting
